The results of liver function tests were not significantly different between the HCV‐positive and HCV‐negative students. Using HCV antibody testing, only 25 students (0.0028%) had positive antibodies among them, 24 students (0.0026%) had HCV RNA positive the study showed none statistically significant higher percentage of HCV infection among males (13 out of 24, 54.2%) than females (11 out of 24, 48.5%), P > 0.05. Results: The mean age of screened students were 18.6 ± 0.39 years. Students with positive HCV antibody testing. Liver function tests and HCV antibody testing were carried out for all students who gave an informed consent HCV‐RNA polymerase chain reaction was performed for Medical examination, ultrasonography, and laboratory investigations were done. Methods: A cross‐sectional serosurvey was conducted including 9049 students. This study aimed at determining the seroprevalence of HCV among the newcomer students of Kafrelsheikh University, Egypt. Genotyping of IL-28B rs8099917 is a useful independent tool for expecting response of Egyptian HCV-infected patients to DAAs.īackground: Hepatitis C virus (HCV) constitutes a global public health problem in Egypt, as it has the highest worldwide prevalence. However, IL-28B rs12979860 alleles did not show any difference.
ICAM-1 rs281437 CT alleles were non significantly more frequent among responders. Regarding response to DAAs treatment, HCV-infected patients with IL-28B rs8099917 GG genotype showed a significantly earlier viral response compared to those carrying TT alleles.
However, IL-28B rs8099917 G allele was more frequent among healthy controls. Allelic discrimination revealed that IL-28B rs12979860 T, IL-28B rs809917 T and ICAM-1 rs281437 C alleles were more frequent among HCV-infected patients (responders or non-responders) than controls. 90% of patients had HCV genotype 4a and the remaining 10% had 4l genotype. IL-28B and ICAM-1 SNPs were evaluated by real-time PCR.ĪLT and AST levels were significantly higher among non-responder HCV infected patients (P = 0.001*). Liver function testing, anti-HCV antibodies, HCV-RNA viral load and HCV genotyping were done. HCV-infected patients were subdivided into responders and non-responders to DAAs. Patients and Methods: Whole blood genomic DNA was extracted from 120 participants (80 HCV-infected patients and 40 healthy volunteers). The aim of the current study was to investigate the impact of IL-28B rs12979860 and rs8099917, and, ICAM-1 rs281437 SNPs on response to treatment with sofosbuvir + Daclatsvir ± Ribavirin, among HCV-infected Egyptian patients. Single nucleotide polymorphisms (SNPs) of IL-28B and/or ICAM-1 could have a role in expecting response of HCV infected patients to direct antiviral agents (DAAs).
0 kommentar(er)
